Enter concentrations and peak areas. The tool performs linear regression, plots linearity, and calculates: LOD = 3.3 × σ / S, LOQ = 10 × σ / S.
| Name Of Organisation |
| Product Name |
| Component Name |
| Potency (%) |
Start with Stock-I (base stock with weight & dilution). You can rename stocks by editing the Stock Name column. Use "+ Add Stock" to add dilution steps. Each dilution can be created from any previous stock by selecting the parent stock from the dropdown. This allows multiple parallel dilutions from the same stock. Concentrations update automatically.
| Dilution-1 | Dilution-2 | ||||
|---|---|---|---|---|---|
| Wt (mg) | Dilution | Conc (µg/mL) | ml Taken | Dilution | Conc (µg/mL) |
| – µg/mL | – µg/mL | ||||
| # | Stock Source | ml Taken | Dilution | Concentration (µg/mL) |
Peak Area-1 (arb. units) |
Peak Area-2 (arb. units) |
Average (arb. units) |
% Level (vs test conc.) |
Delete |
|---|
Tip: Use the arrow keys to move between cells, just like in a spreadsheet. Concentrations are calculated automatically from the selected stock, ml taken, and dilution.
Note: LOD = 3.3 × σ / S • LOQ = 10 × σ / S • σ from residuals ≈ standard deviation of response (ICH Q2).